Anti-hepatoma activity of resveratrol in vitro

World J Gastroenterol. 2002 Feb;8(1):79-81. doi: 10.3748/wjg.v8.i1.79.


Aim: To study the anti-tumor effect of resveratrol alone and the synergistic effects of resveratrol with 5-FU on the growth of H22 cells line in vitro.

Methods: The number of cells was measured by MTT method the morphological changes of H22 cells were investigated under microscopy and electron microscopy examination.

Results: Resveratrol inhibited the growth of hepatoma cells line H22 in a dose- and time-dependent manner, IC50 of the resveratrol on H22 cells was 6.57mg x L(-1),The synergistic anti-tumor effects of resveratrol with 5-FU increased to a greater extent than for H22 cells treated with 5-FU alone (70.2% vs 28.4%) P<0.05 .Under microscope and electron microscope, characteristics of apoptosis such as typical apoptotic bodies were commonly found in tumor cells in the drug-treated groups.

Conclusion: Resveratrol can suppresses the growth of H22 cells in vitro,its anti-tumor activity may occur through the induction of apoptosis.

MeSH terms

  • Antineoplastic Agents, Phytogenic / pharmacology*
  • Apoptosis / drug effects
  • Carcinoma, Hepatocellular*
  • Cell Division / drug effects
  • Humans
  • In Vitro Techniques
  • Liver Neoplasms*
  • Resveratrol
  • Stilbenes / pharmacology*
  • Tumor Cells, Cultured / cytology
  • Tumor Cells, Cultured / drug effects


  • Antineoplastic Agents, Phytogenic
  • Stilbenes
  • Resveratrol