Mushroom-derived preparations in the prevention of H2O2-induced oxidative damage to cellular DNA

Teratog Carcinog Mutagen. 2002;22(2):103-11. doi: 10.1002/tcm.10008.


Aqueous extracts of the sporophores of eight mushroom species were assessed for their ability to prevent H2O2-induced oxidative damage to cellular DNA using the single-cell gel electrophoresis ("Comet") assay. The highest genoprotective effects were obtained with cold (20 degrees C) and hot (100 degrees C) water extracts of Agaricus bisporus and Ganoderma lucidum fruit bodies, respectively. No protective effects were observed with Mushroom Derived Preparations (MDPs) from Flammulina velutipes, Auricularia auricula, Hypsizygus marmoreus, Lentinula edodes, Pleurotus sajor-caju, and Volvariella volvacea. These findings indicate that some edible mushrooms represent a valuable source of biologically active compounds with potential for protecting cellular DNA from oxidative damage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agaricales / chemistry*
  • Comet Assay
  • DNA Damage / drug effects*
  • DNA, Neoplasm / drug effects*
  • DNA, Neoplasm / metabolism
  • Fungal Proteins / pharmacology*
  • Hydrogen Peroxide / toxicity*
  • Mutagenicity Tests
  • Oxidation-Reduction
  • Oxidative Stress / drug effects*
  • Reactive Oxygen Species
  • Tumor Cells, Cultured / drug effects*
  • Tumor Cells, Cultured / metabolism


  • DNA, Neoplasm
  • Fungal Proteins
  • Reactive Oxygen Species
  • Hydrogen Peroxide