Action range of BMP is defined by its N-terminal basic amino acid core
- PMID: 11839272
- DOI: 10.1016/s0960-9822(01)00684-4
Action range of BMP is defined by its N-terminal basic amino acid core
Abstract
During early development, cells receive positional information from neighboring cells to form tissue patterns in initially uniform germ layers. Ligands of the transforming growth factor (TGF-beta) superfamily are known to participate in this pattern formation. In particular, activin has been shown to act as a long-range dorsalizing signal to establish a concentration gradient in Xenopus. In contrast, BMP-2 and BMP-4, other members of the family, appear to influence and induce ventral fates only where they are expressed. This raises a question as to how the action of BMPs is tightly restricted to the region within and around the cells that produce them. Here, we have demonstrated that a basic core of only three amino acids in the N-terminal region of BMP-4 is required for its restriction to the non-neural ectoderm as its expression domain. Our results also suggest that heparan sulfate proteoglycans bind to this basic core and thus play a role in trapping BMP-4. The present study is the first to identify the critical domain of BMP that is responsible for its interaction with the extracellular environment that restricts its diffusion in vivo.
Similar articles
-
Ventral mesoderm induction and patterning by bone morphogenetic protein heterodimers in Xenopus embryos.Mech Dev. 1998 Jun;74(1-2):75-88. doi: 10.1016/s0925-4773(98)00070-7. Mech Dev. 1998. PMID: 9651485
-
Bone morphogenetic proteins.Growth Factors. 2004 Dec;22(4):233-41. doi: 10.1080/08977190412331279890. Growth Factors. 2004. PMID: 15621726 Review.
-
In Xenopus embryos, BMP heterodimers are not required for mesoderm induction, but BMP activity is necessary for dorsal/ventral patterning.Dev Biol. 1999 Dec 1;216(1):29-40. doi: 10.1006/dbio.1999.9496. Dev Biol. 1999. PMID: 10588861
-
Two-step induction of primitive erythrocytes in Xenopus laevis embryos: signals from the vegetal endoderm and the overlying ectoderm.Int J Dev Biol. 2001 Apr;45(2):387-96. Int J Dev Biol. 2001. PMID: 11330858
-
A gradient of BMP activity specifies dorsal-ventral fates in early Xenopus embryos.Semin Cell Dev Biol. 1999 Jun;10(3):319-26. doi: 10.1006/scdb.1999.0308. Semin Cell Dev Biol. 1999. PMID: 10441546 Review.
Cited by
-
Control of tissue homeostasis by the extracellular matrix: Synthetic heparan sulfate as a promising therapeutic for periodontal health and bone regeneration.Periodontol 2000. 2024 Feb;94(1):510-531. doi: 10.1111/prd.12515. Epub 2023 Aug 24. Periodontol 2000. 2024. PMID: 37614159 Review.
-
SMOC-1 interacts with both BMP and glypican to regulate BMP signaling in C. elegans.PLoS Biol. 2023 Aug 17;21(8):e3002272. doi: 10.1371/journal.pbio.3002272. eCollection 2023 Aug. PLoS Biol. 2023. PMID: 37590248 Free PMC article.
-
Switching mechanism from AR to EGFR signaling via 3-O-sulfated heparan sulfate in castration-resistant prostate cancer.Sci Rep. 2023 Jul 18;13(1):11618. doi: 10.1038/s41598-023-38746-x. Sci Rep. 2023. PMID: 37463954 Free PMC article.
-
The role of glycosaminoglycan modification in Hedgehog regulated tissue morphogenesis.Biochem Soc Trans. 2023 Jun 28;51(3):983-993. doi: 10.1042/BST20220719. Biochem Soc Trans. 2023. PMID: 37222258 Free PMC article. Review.
-
Drosophila hedgehog signaling range and robustness depend on direct and sustained heparan sulfate interactions.Front Mol Biosci. 2023 Feb 22;10:1130064. doi: 10.3389/fmolb.2023.1130064. eCollection 2023. Front Mol Biosci. 2023. PMID: 36911531 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
