Small-scale batch crystallization of proteins revisited: an underutilized way to grow large protein crystals

Structure. 2002 Feb;10(2):147-51. doi: 10.1016/s0969-2126(02)00711-6.

Abstract

Growth of high-quality crystals is a major obstacle in many structural investigations. In recent years, the techniques for screening crystals have improved dramatically, whereas the methods for obtaining large crystals have progressed more slowly. This is an important issue since, although many structures can be solved from small crystals with synchrotron radiation, it is far easier to solve and refine structures when strong data is recorded from large crystals. In an effort to improve the size of crystals, a strategy for a small-scale batch method has been developed that in many cases yields far larger crystals than attainable by vapor diffusion.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacterial Proteins / chemistry
  • Crystallization
  • Crystallography / methods*
  • Diffusion
  • Molecular Weight
  • Oils
  • Proteins / chemistry*
  • Proteins / isolation & purification
  • X-Ray Diffraction / methods

Substances

  • Bacterial Proteins
  • Oils
  • Proteins