DNA methylation analysis by MethyLight technology

Methods. 2001 Dec;25(4):456-62. doi: 10.1006/meth.2001.1268.


MethyLight is a sensitive, fluorescence-based real-time PCR technique that is capable of quantitating DNA methylation at a particular locus by using DNA oligonucleotides that anneal differentially to bisulfite-converted DNA according to the methylation status in the original genomic DNA. The use of three oligonucleotides (forward and reverse primers, and interpositioned probe) in MethyLight, any one or more of which can be used for methylation discrimination, allows for a high degree of specificity, sensitivity, and flexibility in methylation detection.

MeSH terms

  • CpG Islands
  • DNA Methylation*
  • DNA Primers / metabolism
  • Humans
  • Models, Genetic
  • Polymerase Chain Reaction / methods*
  • Reverse Transcriptase Polymerase Chain Reaction*
  • Sensitivity and Specificity
  • Sulfites / pharmacology


  • DNA Primers
  • Sulfites
  • sodium bisulfite