Mass fragmentography of 5-hydroxytryptophol and 5-methoxytryptophol in human cerebrospinal fluid

J Chromatogr. 1975 Oct 29;112:523-31. doi: 10.1016/s0021-9673(00)99981-3.


A specific and very sensitive method for the determination of 5-hydroxy-tryptophol (5-HTOL) and 5-methoxytryptophol (5-MTOL) in extracts from human cerebrospinal fluid (CSF) involving the use of mass fragmentography and pentafluoropriopionyl derivatives is described. 5-HTOL and 5-MTOL were determined in human CSF of three patients with leukaemia and from nine patients with neurological disorders. The concentration of free 5-HOT in CSF was in the range of 0.1-33 ng/ml and that of 5-MTOL was 0.3-13.9 ng/ml. For the first time the presence of these compounds in human material has been shown. The concentration of these two alcohols in CSF is markedly lower than the concentration of 5-hydroxyindoleacetic acid. These results suggest that human cerebral 5-hydroxytryptamine is preferentially metabolized to 5-HTOL-hydroxyindoleacetic acid rather than to 5-HTOL and 5-MTOL.

MeSH terms

  • Adolescent
  • Child
  • Chromatography, Gas*
  • Dopamine / metabolism
  • Electrons
  • Female
  • Gas Chromatography-Mass Spectrometry*
  • Humans
  • Hydroxytryptophol / cerebrospinal fluid*
  • Indoles / cerebrospinal fluid*
  • Leukemia / cerebrospinal fluid
  • Male
  • Nervous System Diseases / cerebrospinal fluid
  • Serotonin / metabolism


  • Indoles
  • Hydroxytryptophol
  • Serotonin
  • methoxytryptophol
  • Dopamine