A peripheral nervous system actin-binding protein regulates neurite outgrowth

Eur J Neurosci. 2002 Jan;15(2):281-90. doi: 10.1046/j.0953-816x.2001.01862.x.


Difference cloning has identified a Villin-like mRNA transcript expressed selectively in peripheral sensory and sympathetic neurons. Pervin, the encoded 820-amino acid protein, has 60% identity with Villin and is the rat homologue of Advillin. Coimmunoprecipitation studies demonstrate that Pervin and actin interact in vivo. Transfection of COS-7 epithelial cell lines demonstrates colocalization of epitope-tagged Pervin with green fluorescent protein-actin and results in an increase in process formation. This effect is abolished when the putative actin-bundling headpiece of Pervin is deleted. Biolistic transfection of primary cultures of rat dorsal root ganglion sensory neurons also results in increased neurite outgrowth with FLAG-tagged Pervin. Deletion of the actin-bundling headpiece inhibits normal neurite growth. These data suggest that Pervin may play a significant role in regulating process outgrowth in peripheral neurons through a mechanism that involves the activity of an actin-bundling domain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • COS Cells
  • Carrier Proteins / genetics
  • Conserved Sequence
  • Cytoskeleton / metabolism
  • Ganglia, Spinal / cytology
  • Gene Expression / physiology
  • Green Fluorescent Proteins
  • Indicators and Reagents / metabolism
  • Luminescent Proteins / genetics
  • Microfilament Proteins / genetics*
  • Microfilament Proteins / physiology
  • Molecular Sequence Data
  • Neurites / chemistry*
  • Neurites / physiology*
  • Peripheral Nervous System / chemistry*
  • Peripheral Nervous System / cytology
  • Rats
  • Sequence Homology, Amino Acid


  • AVIL protein, human
  • Avil protein, rat
  • Carrier Proteins
  • Indicators and Reagents
  • Luminescent Proteins
  • Microfilament Proteins
  • villin
  • Green Fluorescent Proteins