Rhythmic expression of ROR beta mRNA in the mice suprachiasmatic nucleus

Neurosci Lett. 2002 Mar 1;320(1-2):13-6. doi: 10.1016/s0304-3940(02)00011-3.

Abstract

The expression of the brain rich orphan nuclear receptor ROR beta (retinoid-related orphan receptor beta) was investigated in the mouse brain by in situ hybridization using antisense cRNA probe. Positive ROR beta mRNA signals were detected in various parts of the brain with high expression in the suprachiasmatic nucleus (SCN). In the SCN, ROR beta mRNA signals showed a peak at early daytime (ZT/CT4) and a trough at early nighttime (ZT/CT16) in both light-dark and constant dark conditions. Light exposure at subjective night did not alter the expression level. These findings suggest that ROR beta is a new member of a transcription factor possibly related to the circadian pacemaking system.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Clocks / physiology*
  • Brain / cytology
  • Brain / metabolism
  • Cell Cycle Proteins
  • Circadian Rhythm / physiology*
  • Down-Regulation / physiology
  • Male
  • Mice
  • Mice, Inbred BALB C / anatomy & histology
  • Mice, Inbred BALB C / metabolism*
  • Motor Activity / physiology
  • Nuclear Proteins / metabolism
  • Nuclear Receptor Subfamily 1, Group F, Member 2
  • Period Circadian Proteins
  • Photic Stimulation
  • RNA, Messenger / metabolism*
  • Receptors, Cell Surface / genetics*
  • Receptors, Cytoplasmic and Nuclear*
  • Suprachiasmatic Nucleus / cytology
  • Suprachiasmatic Nucleus / metabolism*
  • Transcription Factors
  • Up-Regulation / physiology

Substances

  • Cell Cycle Proteins
  • Nuclear Proteins
  • Nuclear Receptor Subfamily 1, Group F, Member 2
  • Per1 protein, mouse
  • Per2 protein, mouse
  • Period Circadian Proteins
  • RNA, Messenger
  • Receptors, Cell Surface
  • Receptors, Cytoplasmic and Nuclear
  • Transcription Factors