Advances in proteome analysis by mass spectrometry

Curr Opin Biotechnol. 2001 Dec;12(6):607-12. doi: 10.1016/s0958-1669(01)00268-3.


Proteome characterization using mass spectrometry is essential for the systematic investigation of biological systems and for the study of gene function. Recent advances in this multifaceted field have occurred in four general areas: protein and peptide separation methodologies; selective labeling chemistries for quantitative measurement of peptide and protein abundances; characterization of post-translational protein modifications; and instrumentation.

Publication types

  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Chromatography, High Pressure Liquid
  • Electrophoresis, Gel, Two-Dimensional
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Mass Spectrometry / instrumentation
  • Mass Spectrometry / methods*
  • Oligonucleotide Array Sequence Analysis
  • Peptides / chemistry
  • Proteins / analysis*
  • Proteins / chemistry*


  • Peptides
  • Proteins