A quantitative analysis of the kinetics of Gal4 activator and effector gene expression in the zebrafish

Mech Dev. 2002 Mar;112(1-2):9-14. doi: 10.1016/s0925-4773(01)00621-9.


Using a temperature-inducible hsp70:Gal4 activator and UAS:myc-notch1a-intra as effector, we determined quantitatively the kinetics of expression of both transgenes and analysed the effects of varying their expressivity on several phenotypic traits in the developing zebrafish. hsp70:Gal4 is transcribed within 15 min after temperature-mediated induction, but Gal4 RNA decays rapidly. The Gal4 protein was found to be quite stable, as functional Gal4, which was detectable 1.5 h after heat shock (HS), persisted for at least 13 h. myc-notch1a-intra RNA is expressed approximately 1.5 h after HS, but unlike the Gal4 RNA, it was found to be very stable; it continues to accumulate during the succeeding 17 h after HS. Fully penetrant phenotypic effects are obtained after a relatively long activator induction with a 30-min HS.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • DNA-Binding Proteins
  • Fungal Proteins / chemistry*
  • Fungal Proteins / metabolism
  • Gene Expression Regulation, Developmental*
  • Hot Temperature
  • In Situ Hybridization
  • Kinetics
  • RNA / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Saccharomyces cerevisiae Proteins*
  • Temperature
  • Time Factors
  • Transcription Factors / chemistry*
  • Transcription Factors / metabolism
  • Zebrafish


  • DNA-Binding Proteins
  • Fungal Proteins
  • GAL4 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • RNA