Yeast Rev1 protein is a G template-specific DNA polymerase

J Biol Chem. 2002 May 3;277(18):15546-51. doi: 10.1074/jbc.M112146200. Epub 2002 Feb 15.


Rev1 protein of Saccharomyces cerevisiae functions with DNA polymerase zeta in mutagenic trans-lesion synthesis. Because of the reported preferential incorporation of a C residue opposite an abasic site, Rev1 has been referred to as a deoxycytidyltransferase. Here, we use steady-state kinetics to examine nucleotide incorporation by Rev1 opposite undamaged and damaged template residues. We show that Rev1 specifically inserts a C residue opposite template G, and it is approximately 25-, 40-, and 400-fold less efficient at inserting a C residue opposite an abasic site, an O(6)-methylguanine, and an 8-oxoguanine lesion, respectively. Rev1 misincorporates G, A, and T residues opposite template G with a frequency of approximately 10(-3) to 10(-4). Consistent with this finding, Rev1 replicates DNA containing a string of Gs in a template-specific manner, but it has a low processivity incorporating 1.6 nucleotides per DNA binding event on the average. From these observations, we infer that Rev1 is a G template-specific DNA polymerase.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • DNA Damage
  • DNA Primers
  • DNA Transposable Elements
  • DNA-Directed DNA Polymerase / metabolism*
  • Fungal Proteins / metabolism*
  • Kinetics
  • Nucleotidyltransferases*
  • Saccharomyces cerevisiae / enzymology*
  • Saccharomyces cerevisiae Proteins*
  • Substrate Specificity
  • Templates, Genetic


  • DNA Primers
  • DNA Transposable Elements
  • Fungal Proteins
  • Saccharomyces cerevisiae Proteins
  • DNA polymerase zeta
  • Nucleotidyltransferases
  • REV1 protein, S cerevisiae
  • DNA-Directed DNA Polymerase