Oxidative stress and antioxidant status in type 1 diabetes mellitus

J Intern Med. 2002 Jan;251(1):69-76. doi: 10.1046/j.1365-2796.2002.00927.x.


Objectives: To test the hypothesis that type 1 diabetes is associated with increased oxidative stress and/or antioxidant status by investigating concentrations of 8-iso-prostaglandin F2alpha (8-iso-PGF2alpha) in urine and plasma and malondialdehyde (MDA) in plasma as indicators of lipid peroxidation in vivo, and antioxidant status in diabetic subjects compared with healthy control subjects.

Design and subjects: Thirty-eight subjects with type 1 diabetes mellitus and 41 healthy age- and sex-matched control subjects were included in the study. Blood and urine samples were obtained and analysed for 8-iso-PGF2alpha with a newly developed radioimmunoassay, as well as for MDA, total antioxidant capacity (TAOC) and serum tocopherol levels.

Results: None of the variables of lipid peroxidation showed any significant difference between the two groups. Similarly, there were no significant correlations between the levels of 8-iso-PGF2alpha or MDA, and degree of glycemic control (HbA1c). Total antioxidant capacity in plasma was 16% lower amongst the subjects with type 1 diabetes than in the control group (P < 0.0005). Lipid corrected levels of alpha-tocopherol in serum were significantly increased in type 1 diabetic subjects (P < 0.05), as were gamma-tocopherol levels (P < 0.005).

Conclusions: In spite of lower total antioxidant defence, our results do not support the oxidative stress hypothesis for type 1 diabetes mellitus. The higher tocopherol levels suggest that no vitamin E supplementation is necessary for subjects with type 1 diabetes mellitus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Case-Control Studies
  • Chromatography, High Pressure Liquid
  • Diabetes Mellitus, Type 1 / blood*
  • Dinoprost* / analogs & derivatives*
  • F2-Isoprostanes / blood*
  • F2-Isoprostanes / urine
  • Female
  • Humans
  • Lipid Peroxidation
  • Male
  • Malondialdehyde / blood*
  • Middle Aged
  • Oxidative Stress*
  • Radioimmunoassay
  • Tocopherols / blood*
  • Vitamin E / blood


  • F2-Isoprostanes
  • Vitamin E
  • 8-epi-prostaglandin F2alpha
  • Malondialdehyde
  • Dinoprost
  • Tocopherols