Molecular simulations of the large conductance mechanosensitive (MscL) channel under mechanical loading

FEBS Lett. 2002 Feb 13;512(1-3):185-90. doi: 10.1016/s0014-5793(02)02254-8.

Abstract

The MscL channel is a mechanosensitive channel which is gated by membrane stress or tension. Here, we describe a series of simulations which apply simulated mechanical stress to a molecular model of the MscL channel using two methods - direct force application to the transmembrane segments, and anisotropic pressure coupling. In the latter simulations, pressures less than that equivalent to a bilayer tension of 12 dyn/cm did not cause the channel to open, while pressures in excess of this value resulted in the channel opening. These results are in approximate agreement with experimental findings.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Computer Simulation
  • Escherichia coli Proteins*
  • Ion Channel Gating
  • Ion Channels / physiology*
  • Models, Molecular
  • Physical Stimulation
  • Protein Conformation
  • Signal Transduction
  • Surface Tension

Substances

  • Escherichia coli Proteins
  • Ion Channels
  • MscL protein, E coli