The characterization of the human Siah-1 promoter(1)

FEBS Lett. 2002 Feb 13;512(1-3):223-6. doi: 10.1016/s0014-5793(02)02265-2.

Abstract

Siah-1, the human homologue of Drosophila seven in absentia, is related to apoptosis and tumor suppression. Although it was reported that the expression of Siah-1 is induced by p53 and p21/WAF1, little is known about the transcriptional regulation of the Siah-1 gene. To investigate the transcriptional regulation, we isolated and sequenced the genomic fragment of the Siah-1 promoter region. The Siah-1 promoter has no typical TATA box or CCAAT box. Transient transfection assays using reporter plasmids in which the promoter region of the Siah-1 gene was deleted or mutated showed that one Sp1 site was responsible for the basal promoter activity. In Northern blotting analysis, the expression of the Siah-1 gene was upregulated by p53, but activation of the reporter plasmid by the p53 co-transfection assay was not shown, suggesting that a p53 responsive element does not exist in the promoter region we examined in this study but might be present in another region.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5' Flanking Region / genetics
  • Base Sequence
  • Binding Sites
  • Cloning, Molecular
  • Down-Regulation
  • Genes, p53
  • Humans
  • Molecular Sequence Data
  • Nuclear Proteins / genetics*
  • Promoter Regions, Genetic*
  • Sp1 Transcription Factor / metabolism
  • Transcriptional Activation
  • Tumor Suppressor Protein p53 / metabolism
  • Ubiquitin-Protein Ligases

Substances

  • Nuclear Proteins
  • Sp1 Transcription Factor
  • Tumor Suppressor Protein p53
  • Ubiquitin-Protein Ligases
  • seven in absentia proteins

Associated data

  • GENBANK/AB072970