Differential expression of WISP-1 and WISP-2 genes in normal and transformed human breast cell lines

Mol Cell Biochem. 2001 Dec;228(1-2):99-104. doi: 10.1023/a:1013338912642.


The transcriptional alterations of specific gene(s) are actively associated with the development of different cancers including breast. The preceding studies of different laboratories documented at least 40 genes that may contribute directly to the genesis of cancer. Using differential display, RT-PCR and DNA sequencing analyses in normal human mammary epithelial cells (HMEC) and various breast tumor cell lines including MCF-7, ZR-75, T-47D and SKBR2, we demonstrated that WISP-1 and WISP-2 genes are differentially transcribed in these cells. WISP-2 mRNA transcription was identified in all 4 tumor derived cell lines, but the mRNA expression was undetected or minimally detected in normal breast epithelial cells. WISP-1 mRNA expression was identified in normal and transformed cell lines. However, the level of expression was higher in different breast tumor cell lines as compared to HMEC. The mRNA expression profiles of WISP genes in normal breast epithelial cells and breast tumor derived cell lines indicated a strong possibility of the involvement of WISP-signaling in the development of human breast tumors, and can be utilized as genetic markers of this disease.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Base Sequence
  • Breast / cytology
  • Breast / metabolism*
  • Breast Neoplasms / genetics*
  • Breast Neoplasms / metabolism
  • Breast Neoplasms / pathology
  • CCN Intercellular Signaling Proteins
  • Cell Transformation, Neoplastic / genetics*
  • Cell Transformation, Neoplastic / metabolism
  • Consensus Sequence
  • DNA Primers / genetics
  • Epithelial Cells / metabolism
  • Female
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic
  • Growth Substances / biosynthesis
  • Growth Substances / genetics*
  • Humans
  • Intercellular Signaling Peptides and Proteins*
  • Intracellular Signaling Peptides and Proteins
  • Molecular Sequence Data
  • Neoplasm Proteins*
  • Oncogene Proteins / biosynthesis
  • Oncogene Proteins / genetics*
  • Proto-Oncogene Proteins
  • RNA, Messenger / metabolism
  • RNA, Neoplasm / genetics
  • RNA, Neoplasm / metabolism
  • Repressor Proteins
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Sequence Homology, Nucleic Acid
  • Transcription Factors / biosynthesis
  • Transcription Factors / genetics*
  • Tumor Cells, Cultured


  • CCN Intercellular Signaling Proteins
  • CCN4 protein, human
  • CCN5 protein, human
  • DNA Primers
  • Growth Substances
  • Intercellular Signaling Peptides and Proteins
  • Intracellular Signaling Peptides and Proteins
  • Neoplasm Proteins
  • Oncogene Proteins
  • Proto-Oncogene Proteins
  • RNA, Messenger
  • RNA, Neoplasm
  • Repressor Proteins
  • Transcription Factors