Induction of global stress response in Saccharomyces cerevisiae cells lacking telomerase

Biochem Biophys Res Commun. 2002 Mar 1;291(3):714-21. doi: 10.1006/bbrc.2002.6509.

Abstract

Cellular senescence is a major intermediate step from healthy cells toward tumor cells. By using microarrays that simultaneously examine the transcription levels of 6,200 Saccharomyces cerevisiae genes, we show that 45 gene transcript levels are increased and 11 are decreased after exposure to telomere shortening and cellular senescence in a telomerase-deficient mutant. About half of the genes that showed increased expression were found induced under stress, consistent with the notion that critical short telomeres cause stress to cells. Surprisingly, the expression level of telomere recombination genes was not altered suggesting that even though recombination is a means to rescue critically short telomeres, its machinery was not controlled by telomere shortening. The expression of telomere-proximal genes was also analyzed. The possibility of induction of a program to cope with cellular senescence and active telomere-telomere recombination is discussed.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cellular Senescence
  • Down-Regulation
  • Gene Deletion
  • Gene Expression Profiling
  • Gene Expression Regulation, Fungal
  • Oligonucleotide Array Sequence Analysis
  • Phenotype
  • RNA, Fungal / biosynthesis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Saccharomyces cerevisiae / enzymology*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins / biosynthesis
  • Saccharomyces cerevisiae Proteins / genetics
  • Telomerase / genetics
  • Telomerase / physiology*
  • Telomere
  • Transcriptional Activation

Substances

  • RNA, Fungal
  • Saccharomyces cerevisiae Proteins
  • Telomerase