Abstract
A karyotype for the Syrian hamster is proposed based on an R-banding pattern. R-bands were obtained by BrdU incorporation into the cells followed by a combined DAPI and propidium iodide staining of the fixed metaphase spreads. In situ hybridisation was performed with two biotinylated 18-mer PNA (peptide nucleic acid) probes complementary to sequences within the 5S rRNA gene. The 5S rRNA gene repeats map to chromosome 6q2. The present PNA-FISH procedure is an abbreviated and simpler version of that previously published.
Copyright 2002 S. Karger AG, Basel
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bromodeoxyuridine / metabolism
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Chromosome Banding*
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Chromosomes / genetics*
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Cricetinae
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In Situ Hybridization, Fluorescence / methods*
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Indoles / metabolism
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Karyotyping
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Mesocricetus / genetics*
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Molecular Probes / genetics
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Molecular Probes / metabolism*
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Peptide Nucleic Acids / genetics
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Peptide Nucleic Acids / metabolism*
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Physical Chromosome Mapping / methods
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Propidium / metabolism
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RNA, Ribosomal, 5S / genetics*
Substances
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Indoles
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Molecular Probes
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Peptide Nucleic Acids
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RNA, Ribosomal, 5S
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Propidium
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DAPI
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Bromodeoxyuridine