Syrian hamster 5S rRNA genes are assigned to 6q2 with PNA-FISH by an R-banded karyotype

Cytogenet Cell Genet. 2001;94(3-4):169-72. doi: 10.1159/000048810.


A karyotype for the Syrian hamster is proposed based on an R-banding pattern. R-bands were obtained by BrdU incorporation into the cells followed by a combined DAPI and propidium iodide staining of the fixed metaphase spreads. In situ hybridisation was performed with two biotinylated 18-mer PNA (peptide nucleic acid) probes complementary to sequences within the 5S rRNA gene. The 5S rRNA gene repeats map to chromosome 6q2. The present PNA-FISH procedure is an abbreviated and simpler version of that previously published.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bromodeoxyuridine / metabolism
  • Chromosome Banding*
  • Chromosomes / genetics*
  • Cricetinae
  • In Situ Hybridization, Fluorescence / methods*
  • Indoles / metabolism
  • Karyotyping
  • Mesocricetus / genetics*
  • Molecular Probes / genetics
  • Molecular Probes / metabolism*
  • Peptide Nucleic Acids / genetics
  • Peptide Nucleic Acids / metabolism*
  • Physical Chromosome Mapping / methods
  • Propidium / metabolism
  • RNA, Ribosomal, 5S / genetics*


  • Indoles
  • Molecular Probes
  • Peptide Nucleic Acids
  • RNA, Ribosomal, 5S
  • Propidium
  • DAPI
  • Bromodeoxyuridine