Polymorphisms in or near tumour necrosis factor (TNF)-gene do not determine levels of endotoxin-induced TNF production

Genes Immun. 2002 Feb;3(1):25-9. doi: 10.1038/sj.gene.6363824.


Innate differences in tumour necrosis factor (TNF) production have been associated with susceptibility for and outcome of inflammatory diseases. Several studies have tried to identify whether polymorphisms in or near the TNF gene or other markers on the short arm of chromosome 6 (6p21) are related to differences in TNF production. Data on these associations are conflicting. Therefore, we conducted a study among 129 healthy individuals in which TNF production was determined upon stimulation with endotoxin in whole blood cultures. TNFa microsatellite, TNF single nucleotide polymorphisms at position +489, -238, -308 and -376 typing was performed. The data revealed that alleles of TNFa microsatellite and carriership of TNF polymorphisms were not related to TNF production. We conclude that the genes determing the differences in endotoxin-induced TNF production have not been yet identified.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Base Sequence
  • Endotoxins / pharmacology*
  • Female
  • HLA-DR Antigens / genetics
  • Haplotypes
  • Heterozygote
  • Humans
  • Male
  • Microsatellite Repeats
  • Middle Aged
  • Netherlands
  • Polymorphism, Genetic*
  • Polymorphism, Single Nucleotide
  • Tumor Necrosis Factor-alpha / biosynthesis*
  • Tumor Necrosis Factor-alpha / genetics*


  • Endotoxins
  • HLA-DR Antigens
  • Tumor Necrosis Factor-alpha