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. 2002 Feb 22;295(5559):1520-3.
doi: 10.1126/science.1066176.

Enzyme Dynamics During Catalysis

Free article

Enzyme Dynamics During Catalysis

Elan Zohar Eisenmesser et al. Science. .
Free article


Internal protein dynamics are intimately connected to enzymatic catalysis. However, enzyme motions linked to substrate turnover remain largely unknown. We have studied dynamics of an enzyme during catalysis at atomic resolution using nuclear magnetic resonance relaxation methods. During catalytic action of the enzyme cyclophilin A, we detect conformational fluctuations of the active site that occur on a time scale of hundreds of microseconds. The rates of conformational dynamics of the enzyme strongly correlate with the microscopic rates of substrate turnover. The present results, together with available structural data, allow a prediction of the reaction trajectory.

Comment in

  • Enzymology. A moving story.
    Falke JJ. Falke JJ. Science. 2002 Feb 22;295(5559):1480-1. doi: 10.1126/science.1069823. Science. 2002. PMID: 11859184 Free PMC article. No abstract available.

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