Novel, chimpanzee serotype 68-based adenoviral vaccine carrier for induction of antibodies to a transgene product

J Virol. 2002 Mar;76(6):2667-75. doi: 10.1128/jvi.76.6.2667-2675.2002.


An E1-deletion-containing adenoviral recombinant based on the chimpanzee serotype 68 (AdC68) was developed to express the rabies virus glycoprotein. Mice immunized with this construct ( developed antibodies to rabies virus and remained resistant to challenge with an otherwise lethal dose of rabies virus. In naïve mice immunized intranasally, the rabies virus-specific antibody responses elicited by were comparable with regard to both titers and isotype profiles to those induced by an adenoviral recombinant based on human serotype 5 (Adhu5) expressing the same transgene product. In contrast, subcutaneous immunization with the vaccine resulted in markedly lower antibody responses to the rabies virus glycoprotein than the corresponding Adhu5 vaccine. Antibodies from mice were strongly biased towards the immunoglobulin G2a isotype. The antibody response to the rabies virus glycoprotein presented by was severely compromised in animals preexposed to the homologous adenovirus. In contrast, the rabies virus-specific antibody response to the vaccine was at most marginally affected by preexisting immunity to common human adenovirus serotypes, such as 2, 4, 5, 7, and 12. This novel vaccine carrier thus offers a distinct advantage over adenoviral vaccines based on common human serotypes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenoviruses, Simian / classification
  • Adenoviruses, Simian / genetics*
  • Animals
  • Antibodies, Viral / biosynthesis*
  • Antibodies, Viral / immunology
  • Antigens, Viral*
  • B-Lymphocytes / immunology
  • Cell Line
  • Female
  • Genetic Vectors*
  • Glycoproteins / administration & dosage
  • Glycoproteins / genetics
  • Glycoproteins / immunology*
  • Humans
  • Mice
  • Mice, Inbred C3H
  • Rabies / prevention & control
  • Rabies Vaccines / administration & dosage
  • Rabies Vaccines / immunology*
  • Rabies virus / immunology
  • Serotyping
  • Transgenes*
  • Vaccination
  • Viral Envelope Proteins / administration & dosage
  • Viral Envelope Proteins / genetics
  • Viral Envelope Proteins / immunology*


  • Antibodies, Viral
  • Antigens, Viral
  • Glycoproteins
  • Rabies Vaccines
  • Viral Envelope Proteins
  • glycoprotein G, Rabies virus