Chemical incorporation of 1-methyladenosine into oligonucleotides

Nucleic Acids Res. 2002 Mar 1;30(5):1124-31. doi: 10.1093/nar/30.5.1124.

Abstract

The base moiety of 1-N-methyladenosine can be protected with a chloroacetyl group for incorporation of this modified nucleoside into DNA and RNA. Carefully controlled anhydrous conditions are needed for deprotection of the oligonucleotides.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine / analogs & derivatives*
  • Adenosine / chemistry*
  • Hot Temperature
  • Mass Spectrometry
  • Nuclear Magnetic Resonance, Biomolecular
  • Nucleic Acid Denaturation
  • Oligodeoxyribonucleotides / chemical synthesis*
  • Oligoribonucleotides / chemical synthesis*
  • Organophosphorus Compounds / chemical synthesis

Substances

  • Oligodeoxyribonucleotides
  • Oligoribonucleotides
  • Organophosphorus Compounds
  • phosphoramidite
  • 1-methyladenosine
  • Adenosine