Measles Virus Matrix Protein Is Not Cotransported With the Viral Glycoproteins but Requires Virus Infection for Efficient Surface Targeting

Virus Res. 2002 Feb 26;83(1-2):1-12. doi: 10.1016/s0168-1702(01)00379-3.

Abstract

As we have shown earlier, the measles virus (MV) glycoproteins H and F are expressed on both, the apical and the basolateral membrane of polarized Madin-Darby canine kidney cells. In contrast to the glycoproteins, we found the viral matrix protein (M) to accumulate selectively at the apical plasma membrane of MV-infected cells. M did not colocalize with the glycoproteins at basolateral membranes of polarized cells indicating an independent surface transport mechanism. Analysis of infected cells treated with monensin supported this view. When H and F were retained in the medial Golgi by monensin treatment, M did not accumulate in this cellular compartment. To elucidate the subcellular transport mechanism of the cytosolic M protein, M was expressed in the absence of other viral proteins. Flotation analysis demonstrated that most of the M protein coflotated in infected or in M-transfected cells with cellular membranes. Thus, the M protein possesses the intrinsic ability to bind to lipid membranes. Unexpectedly, plasmid-encoded M protein was rarely found to accumulate at surface membranes. Although cotransport with the viral glycoproteins was not needed, M transport to the plasma membrane required a component only provided in MV-infected cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Transport
  • Cell Line
  • Cell Membrane / metabolism
  • Dogs
  • Glycoproteins / metabolism*
  • Measles virus / metabolism*
  • Measles virus / physiology
  • Viral Fusion Proteins / metabolism*
  • Viral Matrix Proteins / metabolism*

Substances

  • Glycoproteins
  • Viral Fusion Proteins
  • Viral Matrix Proteins