Effect of previous or simultaneous immunization with canarypox expressing cytomegalovirus (CMV) glycoprotein B (gB) on response to subunit gB vaccine plus MF59 in healthy CMV-seronegative adults

J Infect Dis. 2002 Mar 1;185(5):686-90. doi: 10.1086/339003. Epub 2002 Feb 6.

Abstract

Development of a vaccine for prevention of congenital cytomegalovirus (CMV) disease is a priority. This study evaluated a "prime-boost" strategy by comparing the safety and immunogenicity of 3 doses of subunit CMV glycoprotein B (gB) vaccine plus MF59 (a squalene-in-water emulsion), 2 doses of a canarypox recombinant vaccine expressing CMVgB (ALVAC-CMVgB) followed by 2 doses of the subunit gB vaccine, 3 doses of both vaccines administered concomitantly, and placebo in 105 healthy, CMV-seronegative adults. Systemic adverse events were rare, but local reactions were common in all groups. After the first subunit vaccination, neutralizing antibody titers in the prime-boost group were comparable to those in subjects receiving 2 subunit vaccinations, indicating a priming effect of ALVAC-CMVgB. However, after the final dose, antibody and cell-mediated immune responses were not significantly different among the groups. All 3 vaccine regimens induced high-titer antibody and lymphoproliferative responses, but no benefit for priming or simultaneous vaccination was detected.

Publication types

  • Clinical Trial
  • Randomized Controlled Trial
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adjuvants, Immunologic / administration & dosage
  • Adolescent
  • Adult
  • Antibodies, Viral / blood*
  • Cytomegalovirus Infections / prevention & control*
  • Cytomegalovirus Vaccines / administration & dosage
  • Cytomegalovirus Vaccines / immunology*
  • Humans
  • Immunization Schedule
  • Immunization, Secondary
  • Lymphocyte Activation*
  • Middle Aged
  • Polysorbates / administration & dosage
  • Squalene / administration & dosage
  • Squalene / immunology
  • Vaccination
  • Vaccines, Subunit / administration & dosage
  • Vaccines, Subunit / immunology*
  • Vaccines, Synthetic / administration & dosage
  • Vaccines, Synthetic / immunology
  • Viral Envelope Proteins / genetics
  • Viral Envelope Proteins / immunology*
  • Viral Envelope Proteins / metabolism
  • Viral Vaccines / administration & dosage
  • Viral Vaccines / genetics
  • Viral Vaccines / immunology

Substances

  • ALVAC vaccine
  • Adjuvants, Immunologic
  • Antibodies, Viral
  • Cytomegalovirus Vaccines
  • MF59 oil emulsion
  • Polysorbates
  • Vaccines, Subunit
  • Vaccines, Synthetic
  • Viral Envelope Proteins
  • Viral Vaccines
  • glycoprotein B, Simplexvirus
  • Squalene