OBJECTIVE: To present data on episodes of fungemia in a Danish tertiary-care university hospital admitting all types of patients and to compare the data with previous findings from the same hospital. METHODS: Retrospective identification of episodes of fungemia from 1989 to 1994 and collection of data from computerized files at the Clinical Microbiology Department at Rigshospitalet and the Mycology Reference Laboratory at Statens Seruminstitut. RESULTS: The incidence of fungemia increased gradually from 19 episodes in 1989 to 57 episodes in 1994. An earlier report from the same hospital showed 20 to 25 episodes of fungemia per year between 1984 and 1988. Candida albicans was the dominating species during both periods, accounting for 73% of isolates during 1984 to 1988 and 67% during 1989 to 1994. However, in the hematology department where fluconazole has been used extensively, C. albicans constituted 47% of isolates with Candida krusei and Candida glabrata comprising 25%. CONCLUSIONS: The incidence of fungemia in our tertiary-care hospital has increased threefold from 1989 to 1994. Candida albicans was the dominating cause of fungemia but, in the hematology department, this yeast accounted for less than half of the isolates during the same time period.