Characterization of a novel gene up-regulated during anoxia exposure in the marine snail, Littorina littorea

Gene. 2002 Jan 23;283(1-2):145-54. doi: 10.1016/s0378-1119(01)00873-3.


Gene expression was investigated during anoxia exposure in the marine snail, Littorina littorea. Differential screening of a cDNA library made from hepatopancreas of anoxic L. littorea yielded a 525 bp clone coding for the novel gene kvn. The deduced amino acid sequence of the KVN protein contained 99 amino acid residues with a predicted molecular weight of 12 kDa and showed an N-terminal secretory signal. Analysis of hepatopancreas samples over a time course of anoxia exposure showed a maximum increase in transcript levels of 5.8-fold after 48 h relative to normoxic animals, with a subsequent decrease in transcript levels during normoxic recovery. Nuclear run-off assays confirmed the observed transcriptional up-regulation of kvn during anoxia. Organ culture experiments were performed to determine a possible pathway of up-regulation of kvn, with data indicating a putative role for cGMP in signal transduction. Profiles of ribosome distribution in polysomes versus monosomes revealed a reduction in the polysome peak during anoxia and a shift in the position of kvn transcripts to association with the lower density polysome/higher density monosome region. The data suggest that the kvn transcript is both transcribed and translated during anoxia, indicating a possible significant role for the KVN protein in the survival of anoxia by L. littorea.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • Electron Transport / genetics
  • Gene Expression Regulation
  • Hypoxia / physiopathology*
  • Molecular Sequence Data
  • Organ Culture Techniques
  • Polyribosomes / genetics
  • Proteins / genetics*
  • RNA / genetics
  • RNA / metabolism
  • Sequence Analysis, DNA
  • Snails / genetics*
  • Transcription, Genetic
  • Up-Regulation


  • DNA, Complementary
  • Proteins
  • RNA

Associated data

  • GENBANK/AY046925