Deltex1 redirects lymphoid progenitors to the B cell lineage by antagonizing Notch1

Immunity. 2002 Feb;16(2):231-43. doi: 10.1016/s1074-7613(02)00271-6.


Notch1 signaling drives T cell development at the expense of B cell development from a common precursor, an effect that is dependent on a C-terminal Notch1 transcriptional activation domain. The function of Deltex1, initially identified as a positive modulator of Notch function in a genetic screen in Drosophila, is poorly understood. We now demonstrate that, in contrast to Notch1, enforced expression of Deltex1 in hematopoietic progenitors results in B cell development at the expense of T cell development in fetal thymic organ culture and in vivo. Consistent with these effects, Deltex1 antagonizes Notch1 signaling in transcriptional reporter assays by inhibiting coactivator recruitment. These data suggest that a balance of inductive Notch1 signals and inhibitory signals mediated through Deltex1 and other modulators regulate T-B lineage commitment.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3T3 Cells
  • Animals
  • B-Lymphocytes / cytology*
  • Basic Helix-Loop-Helix Transcription Factors
  • Bone Marrow Cells / cytology
  • Carrier Proteins*
  • Cell Differentiation
  • Cell Line, Transformed
  • Cell Lineage
  • DNA-Binding Proteins*
  • Gene Expression
  • Hematopoietic Stem Cells / cytology*
  • Humans
  • Liver / cytology
  • Liver / embryology
  • Lymphocytes / cytology
  • Membrane Proteins / metabolism*
  • Mice
  • Organ Culture Techniques
  • Protein Biosynthesis*
  • Proteins / genetics
  • Receptor, Notch1
  • Receptors, Cell Surface*
  • Recombinant Fusion Proteins / biosynthesis
  • Recombinant Fusion Proteins / genetics
  • Signal Transduction*
  • T-Lymphocytes / cytology*
  • Thymus Gland / cytology
  • Thymus Gland / embryology
  • Transcription Factors / metabolism
  • Transcriptional Activation
  • Tumor Cells, Cultured


  • Basic Helix-Loop-Helix Transcription Factors
  • Carrier Proteins
  • DNA-Binding Proteins
  • Membrane Proteins
  • NOTCH1 protein, human
  • Notch1 protein, mouse
  • Proteins
  • Receptor, Notch1
  • Receptors, Cell Surface
  • Recombinant Fusion Proteins
  • TCF3 protein, human
  • Transcription Factors
  • deltex protein, vertebrate