CD36, serves as a receptor for advanced glycation endproducts (AGE)

J Diabetes Complications. Jan-Feb 2002;16(1):56-9. doi: 10.1016/s1056-8727(01)00208-2.


Interaction of advanced glycation endproducts (AGE) with AGE receptors induces several cellular phenomena relating potentially to diabetic complications. Five AGE receptors identified so far are receptor for AGE (RAGE), 80 K-H, OST-48, galectin-3, and macrophage scavenger receptor, types I and II (SR-A) [Eur. J. Biochem. 230 (1995) 408; Nature 386 (1997) 292.]. Since SR-A is known to belong to the class A scavenger receptor family and the scavenger receptor collectively represents a family of multiligand lipoprotein receptors, it is possible that CD36 belonging to class B scavenger receptor family (SR-B) can recognize AGE proteins as a ligand. This was tested in the present study at the cellular level by using Chinese hamster ovary (CHO) cells overexpressing human CD36 (CHO-CD36 cells). 125I-AGE-bovine serum albumin (BSA) was endocytosed in a dose-dependent fashion and underwent lysosomal degradation by CHO-CD36, but not wild-type CHO cells. Endocytic uptake of 125I-AGE-BSA by these cells was inhibited 50% by oxidized low-density lipoprotein (Ox-LDL) and 60% by FA6-152, an anti-CD36 antibody inhibiting cellular binding of Ox-LDL. Our results indicate that CD36 expressed by these cells mediates endocytic uptake and subsequent intracellular degradation of AGE proteins. Since CD36 is one of the major Ox-LDL receptors and is up-regulated in macrophage- and smooth muscle cell-derived foam cells in human atherosclerotic lesions, the present results suggest that, like Ox-LDL, AGE proteins generated in situ are recognized by CD36, which might contribute to the pathogenesis of diabetic macrovascular complications.

MeSH terms

  • Animals
  • Biological Transport
  • CD36 Antigens / physiology*
  • CHO Cells
  • Cricetinae
  • Endocytosis
  • Glycation End Products, Advanced / metabolism*
  • Humans
  • Membrane Proteins*
  • Receptor for Advanced Glycation End Products
  • Receptors, Immunologic / physiology*
  • Receptors, Lipoprotein*
  • Receptors, Scavenger
  • Recombinant Proteins / metabolism
  • Scavenger Receptors, Class A
  • Scavenger Receptors, Class B
  • Transfection


  • CD36 Antigens
  • Glycation End Products, Advanced
  • Membrane Proteins
  • Receptor for Advanced Glycation End Products
  • Receptors, Immunologic
  • Receptors, Lipoprotein
  • Receptors, Scavenger
  • Recombinant Proteins
  • SCARB1 protein, human
  • Scarb1 protein, mouse
  • Scavenger Receptors, Class A
  • Scavenger Receptors, Class B