Wax ester production from n-alkanes by Acinetobacter sp. strain M-1: ultrastructure of cellular inclusions and role of acyl coenzyme A reductase

Appl Environ Microbiol. 2002 Mar;68(3):1192-5. doi: 10.1128/AEM.68.3.1192-1195.2002.


Acinetobacter sp. strain M-1 accumulated a large amount of wax esters from an n-alkane under nitrogen-limiting conditions. Under the optimized conditions with n-hexadecane as the substrate, the amount of hexadecyl hexadecanoate in the cells reached 0.17 g/g of cells (dry weight). Electron microscopic analysis revealed that multilayered disk-shaped intracellular inclusions were formed concomitant with wax ester formation. The contribution of acyl-CoA reductase to wax ester synthesis was evaluated by gene disruption analysis.

MeSH terms

  • Acinetobacter / genetics
  • Acinetobacter / metabolism
  • Acinetobacter / ultrastructure*
  • Aldehyde Oxidoreductases / genetics
  • Aldehyde Oxidoreductases / metabolism*
  • Alkanes / metabolism*
  • Esters / metabolism
  • Gene Deletion
  • Inclusion Bodies / ultrastructure*
  • Microscopy, Electron
  • Molecular Sequence Data
  • Waxes / metabolism*


  • Alkanes
  • Esters
  • Waxes
  • Aldehyde Oxidoreductases
  • hexadecanal dehydrogenase (acylating)

Associated data

  • GENBANK/AB070446