MAP Kinase Signalling Cascade in Arabidopsis Innate Immunity

Nature. 2002 Feb 28;415(6875):977-83. doi: 10.1038/415977a.

Abstract

There is remarkable conservation in the recognition of pathogen-associated molecular patterns (PAMPs) by innate immune responses of plants, insects and mammals. We developed an Arabidopsis thaliana leaf cell system based on the induction of early-defence gene transcription by flagellin, a highly conserved component of bacterial flagella that functions as a PAMP in plants and mammals. Here we identify a complete plant MAP kinase cascade (MEKK1, MKK4/MKK5 and MPK3/MPK6) and WRKY22/WRKY29 transcription factors that function downstream of the flagellin receptor FLS2, a leucine-rich-repeat (LRR) receptor kinase. Activation of this MAPK cascade confers resistance to both bacterial and fungal pathogens, suggesting that signalling events initiated by diverse pathogens converge into a conserved MAPK cascade.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Arabidopsis / enzymology*
  • Arabidopsis / genetics
  • Arabidopsis / immunology*
  • Arabidopsis Proteins / metabolism
  • Botrytis / physiology
  • Feedback, Physiological
  • Flagellin / metabolism
  • Gene Expression Regulation, Plant
  • MAP Kinase Kinase Kinases / metabolism
  • MAP Kinase Signaling System*
  • Mitogen-Activated Protein Kinase Kinases / metabolism
  • Mitogen-Activated Protein Kinases / metabolism
  • Plant Diseases / microbiology
  • Protein Kinases / metabolism
  • Protoplasts
  • Pseudomonas / physiology
  • Transcription, Genetic

Substances

  • Arabidopsis Proteins
  • Flagellin
  • Protein Kinases
  • FLS2 protein, Arabidopsis
  • Mitogen-Activated Protein Kinases
  • MAP Kinase Kinase Kinases
  • Mitogen-Activated Protein Kinase Kinases