MEC-2 regulates C. elegans DEG/ENaC channels needed for mechanosensation

Nature. 2002 Feb 28;415(6875):1039-42. doi: 10.1038/4151039a.


Touch sensitivity in animals relies on nerve endings in the skin that convert mechanical force into electrical signals. In the nematode Caenorhabditis elegans, gentle touch to the body wall is sensed by six mechanosensory neurons that express two amiloride-sensitive Na+ channel proteins (DEG/ENaC). These proteins, MEC-4 and MEC-10, are required for touch sensation and can mutate to cause neuronal degeneration. Here we show that these mutant or 'd' forms of MEC-4 and MEC-10 produce a constitutively active, amiloride-sensitive ionic current when co-expressed in Xenopus oocytes, but not on their own. MEC-2, a stomatin-related protein needed for touch sensitivity, increased the activity of mutant channels about 40-fold and allowed currents to be detected with wild-type MEC-4 and MEC-10. Whereas neither the central, stomatin-like domain of MEC-2 nor human stomatin retained the activity of full-length MEC-2, both produced amiloride-sensitive currents with MEC-4d. Our findings indicate that MEC-2 regulates MEC-4/MEC-10 ion channels and raise the possibility that similar ion channels may be formed by stomatin-like proteins and DEG/ENaC proteins that are co-expressed in both vertebrates and invertebrates. Some of these channels may mediate mechanosensory responses.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amiloride / pharmacology
  • Animals
  • Blood Proteins / chemistry
  • Caenorhabditis elegans
  • Caenorhabditis elegans Proteins*
  • Electrophysiology
  • Epithelial Sodium Channels
  • Escherichia coli
  • Genes, Helminth
  • Helminth Proteins / genetics
  • Helminth Proteins / physiology*
  • Humans
  • Ion Channels / genetics
  • Ion Channels / physiology*
  • Mechanoreceptors / physiology*
  • Membrane Proteins / chemistry
  • Membrane Proteins / genetics
  • Membrane Proteins / physiology*
  • Mutation
  • Oocytes
  • Protein Structure, Tertiary
  • Recombinant Proteins
  • Sodium / metabolism
  • Sodium Channels / genetics
  • Sodium Channels / physiology*
  • Touch / physiology*
  • Xenopus


  • Blood Proteins
  • Caenorhabditis elegans Proteins
  • Epithelial Sodium Channels
  • Helminth Proteins
  • Ion Channels
  • MEC-2 protein, C elegans
  • Mec-4 protein, C elegans
  • Membrane Proteins
  • Recombinant Proteins
  • STOM protein, human
  • Sodium Channels
  • MEC-10 protein, C elegans
  • Amiloride
  • Sodium