Role of beta3-adrenergic receptors in the action of a tumour lipid mobilizing factor

Br J Cancer. 2002 Feb 1;86(3):424-8. doi: 10.1038/sj.bjc.6600086.


Induction of lipolysis in murine white adipocytes, and stimulation of adenylate cyclase in adipocyte plasma membranes, by a tumour-produced lipid mobilizing factor, was attenuated by low concentrations (10(-7)--10(-5)M) of the specific beta3-adrenoceptor antagonist SR59230A. Lipid mobilizing factor (250 nM) produced comparable increases in intracellular cyclic AMP in CHOK1 cells transfected with the human beta3-adrenoceptor to that obtained with isoprenaline (1 nM). In both cases cyclic AMP production was attenuated by SR59230A confirming that the effect is mediated through a beta3-adrenoceptor. A non-linear regression analysis of binding of lipid mobilizing factor to the beta3-adrenoceptor showed a high affinity binding site with a Kd value 78 +/- 45 nM and a B(max) value (282 +/- 1 fmole mg protein(-1)) comparable with that of other beta3-adrenoceptor agonists. These results suggest that lipid mobilizing factor induces lipolysis through binding to a beta3-adrenoceptor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenylyl Cyclases / metabolism
  • Adipocytes / cytology
  • Adipocytes / metabolism
  • Animals
  • Appetite Depressants / metabolism*
  • CHO Cells
  • Cricetinae
  • Cyclic AMP / metabolism
  • Epididymis / cytology
  • Humans
  • Lipid Mobilization / physiology*
  • Lipolysis
  • Male
  • Mice
  • Mice, Inbred Strains
  • Pancreatic Neoplasms / metabolism
  • Pancreatic Neoplasms / physiopathology*
  • Pancreatic Neoplasms / urine
  • Peptides / metabolism*
  • Propranolol / pharmacology
  • Receptors, Adrenergic, beta-3 / drug effects
  • Receptors, Adrenergic, beta-3 / physiology*
  • Weight Loss / physiology*


  • Appetite Depressants
  • Peptides
  • Receptors, Adrenergic, beta-3
  • lipid mobilizing substance
  • Propranolol
  • Cyclic AMP
  • Adenylyl Cyclases