"De novo" sequencing of peptides recovered from in-gel digested proteins by nanoelectrospray tandem mass spectrometry

Mol Biotechnol. 2002 Jan;20(1):107-18. doi: 10.1385/mb:20:1:107.


Proteins separated by one-dimensional or two-dimensional gel electrophoresis can be digested in-gel with trypsin and the recovered peptides can be sequenced de novo using triple quadrupole or hybrid quadrupole time-of-flight instruments equipped with a nanoelectrospray ion source. The peptide sequences determined provide useful information for identification of proteins by homology searching for cloning of the cognate genes by PCR based approaches.

MeSH terms

  • Animals
  • Humans
  • Mass Spectrometry / methods*
  • Oxygen Isotopes / metabolism
  • Peptide Fragments / analysis*
  • Peptide Fragments / metabolism
  • Sequence Analysis, Protein / methods*
  • Trypsin / metabolism


  • Oxygen Isotopes
  • Peptide Fragments
  • Trypsin