Optimal serological tests for the detection of Helicobacter pylori infection in the Chinese population

Aliment Pharmacol Ther. 2002 Mar;16(3):521-6. doi: 10.1046/j.1365-2036.2002.01176.x.


Background: Numerous serological tests for the detection of Helicobacter pylori infection have been developed. However, many perform poorly when evaluated in the Chinese population.

Aim: To search for optimal serological tests for the detection of H. pylori infection in Chinese patients.

Methods: Consecutive dyspeptic patients referred for upper endoscopy were recruited. During endoscopy, gastric biopsies were taken for the CLOtest and histological examination. Patients were then given a 13C-urea breath test. Sera were used to test for H. pylori infection, employing three commercial enzyme-linked immunosorbent assay kits (pylori DTect, HP IgG and GAP IgG). Results were compared with the gold standard defined by the CLOtest, histology and 13C-urea breath test.

Results: Among the 142 patients (47 male, 95 female; mean age, 49 years) recruited, 81 (57%) were H. pylori-positive, 57 (40%) were H. pylori-negative and four (3%) were defined to be indeterminate. Using a self-defined cut-off value after calculation, the best accuracies for the pylori DTect, HP IgG and GAP IgG tests were 97%, 91% and 80%, respectively.

Conclusions: The pylori DTect test is an optimal serological test for the detection of H. pylori infection in Hong Kong Chinese patients. The HP IgG test may be used as an alternative.

Publication types

  • Clinical Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Breath Tests / methods
  • Carbon Isotopes
  • China
  • Dyspepsia / diagnosis
  • Dyspepsia / immunology
  • Dyspepsia / microbiology
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Helicobacter Infections / diagnosis*
  • Helicobacter Infections / immunology*
  • Helicobacter Infections / microbiology
  • Helicobacter pylori / immunology*
  • Helicobacter pylori / isolation & purification*
  • Humans
  • Immunoglobulin G / analysis
  • Immunoglobulin G / immunology
  • Male
  • Middle Aged
  • Predictive Value of Tests
  • Sensitivity and Specificity
  • Serologic Tests / methods*
  • Urea / analysis


  • Carbon Isotopes
  • Immunoglobulin G
  • Urea