Quantification and Duration of Viraemia During Hepatitis A Infection as Determined by Real-Time RT-PCR

J Viral Hepat. 2002 Mar;9(2):101-6. doi: 10.1046/j.1365-2893.2002.00336.x.

Abstract

Human transmission studies and molecular techniques have provided evidence that transient viraemia occurs during infection with hepatitis A virus (HAV). However, the duration of its presence and levels during the phases of clinical disease and convalescence has not yet been well studied in human patients. Real-time RT-PCR techniques are increasingly used to quantify RNA viruses for diagnosis and/or research purposes. We have optimized a one-step RT-PCR that contains a dual-labelled fluorogenic probe to quantify the 5' noncoding region (5' NCR) of HAV. This method has a dynamic range (5-5 x 10(6) copies). The coefficient of regression of the standard curve was, on average 0.978. Intra-assay CVs% varied from 6.1% to 0.98%, and interassay CVs% from 6.46% to 2.1%. In the currently reported study 41 HAV IgM positive serum samples and 200 serum samples from healthy blood donors were tested by the quantitative RT-PCR method. The mean values on the first day of diagnosis found was 6.38 x 10(5) copies/mL. In a longitudinal study, viraemia persisted for an average of 60 days after clinical onset. These results show that viraemia in HAV infection lasts for many weeks.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Child
  • Female
  • Hepatitis A / virology*
  • Hepatitis A Virus, Human / isolation & purification*
  • Humans
  • Male
  • Middle Aged
  • RNA, Viral / blood
  • Reverse Transcriptase Polymerase Chain Reaction
  • Time Factors
  • Viremia / virology*

Substances

  • RNA, Viral