Direct flight muscles in Drosophila develop from cells with characteristics of founders and depend on DWnt-2 for their correct patterning

Dev Biol. 2002 Mar 15;243(2):312-25. doi: 10.1006/dbio.2002.0572.

Abstract

The direct flight muscles (DFMs) of Drosophila allow for the fine control of wing position necessary for flight. In DWnt-2 mutant flies, certain DFMs are either missing or fail to attach to the correct epithelial sites. Using a temperature-sensitive allele, we show that DWnt-2 activity is required only during pupation for correct DFM patterning. DWnt-2 is expressed in the epithelium of the wing hinge primordium during pupation. This expression is in the vicinity of the developing DFMs, as revealed by expression of the muscle founder cell-specific gene dumbfounded in DFM precursors. The observation that a gene necessary for embryonic founder cell function is expressed in the DFM precursors suggests that these cells may have a similar founder cell role. Although the expression pattern of DWnt-2 suggests that it could influence epithelial cells to differentiate into attachment sites for muscle, the expression of stripe, a transcription factor necessary for epithelial cells to adopt an attachment cell fate, is unaltered in the mutant. Ectopic expression of DWnt-2 in the wing hinge during pupation can also create defects in muscle patterning without alterations in stripe expression. We conclude that DWnt-2 promotes the correct patterning of DFMs through a mechanism that is independent of the attachment site differentiation initiated by stripe.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • DNA-Binding Proteins / physiology
  • Drosophila Proteins / deficiency
  • Drosophila Proteins / genetics
  • Drosophila Proteins / physiology*
  • Drosophila melanogaster / genetics
  • Drosophila melanogaster / growth & development
  • Drosophila melanogaster / ultrastructure
  • Ectoderm / physiology
  • Epithelial Cells / cytology
  • Epithelial Cells / metabolism
  • Flight, Animal
  • Gene Expression Regulation, Developmental
  • Larva
  • Mesoderm / physiology
  • Microscopy, Electron, Scanning
  • Morphogenesis / genetics
  • Muscles / cytology*
  • Proto-Oncogene Proteins / deficiency
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins / physiology*
  • Pupa
  • Stem Cells / cytology
  • Transcription Factors / physiology
  • Wings, Animal / growth & development*
  • Wings, Animal / ultrastructure
  • Wnt2 Protein

Substances

  • DNA-Binding Proteins
  • Drosophila Proteins
  • Proto-Oncogene Proteins
  • Transcription Factors
  • Wnt2 Protein
  • Wnt2 protein, Drosophila
  • sr protein, Drosophila