Abstract
In Arabidopsis, SHY2 encodes IAA3, a member of the auxin-induced Aux/IAA family. Gain-of-function mutations in SHY2/IAA3 cause enlarged cotyledons, short hypocotyls, and altered auxin-regulated root development. Here we show that the gain-of-function mutation shy2-2 decreases both the induction and repression of auxin-regulated genes, suggesting that SHY2/IAA3 acts as a negative regulator in auxin signaling. shy2-2 affects auxin induction of many previously characterized primary response genes, implying that it might repress primary auxin responses. In addition, shy2-2 also affects expression of multiple auxin-nonresponsive genes. Light regulates expression of SHY2/IAA3, suggesting a possible link between light and auxin response pathways.
Publication types
-
Comparative Study
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Arabidopsis / drug effects
-
Arabidopsis / genetics*
-
Arabidopsis Proteins*
-
Blotting, Northern
-
Coenzyme A Ligases / genetics
-
Cotyledon / drug effects
-
Cotyledon / growth & development
-
Gene Expression Regulation, Plant / drug effects
-
Gene Expression Regulation, Plant / radiation effects
-
Hypocotyl / drug effects
-
Hypocotyl / growth & development
-
Indoleacetic Acids / pharmacology*
-
Light
-
Mutation
-
Nuclear Proteins / genetics*
-
Plant Growth Regulators / pharmacology*
-
Plant Proteins / genetics
-
Plant Roots / drug effects
-
Plant Roots / growth & development
-
Signal Transduction
-
Soybean Proteins*
Substances
-
Arabidopsis Proteins
-
GH3 protein, soybean
-
Indoleacetic Acids
-
Nuclear Proteins
-
Plant Growth Regulators
-
Plant Proteins
-
SAUR protein, Raphanus sativus
-
SHY2 protein, Arabidopsis
-
Soybean Proteins
-
indoleacetic acid
-
ACS6 protein, Brassica napus
-
Coenzyme A Ligases