Comparison of IgE antibody concentrations to pyromellitic dianhydride-modified laminin and human serum albumin in sera of exposed workers with respiratory complaints

Int Arch Allergy Immunol. 2002 Jan;127(1):79-81. doi: 10.1159/000048172.


Background: Acid anhydrides are used in a variety of industrial branches because of their highly reactive anhydride groups. Adverse effects of anhydride exposure include toxic/irritative effects as well as IgE- and IgG-mediated respiratory disorders. It was the aim of this study to examine the usefulness of conjugates of pyromellitic dianhydride (PMDA) and laminin, a respiratory tract protein, for the in vitro diagnosis of sensitization to PMDA.

Methods: Sera of PMDA-exposed workers (n = 9) and nonexposed controls (n = 8) were tested with an enzyme allergosorbent test (EAST). Human laminin and human serum albumin (HSA) were derivatized with PMDA and used as solid-phase in a commercial assay.

Results: Seven out of 9 exposed workers revealed elevated IgE antibody concentrations (>0.35 kU/l) against laminin-PMDA whereas 5 of 9 subjects had elevated IgE antibody concentrations to HSA-PMDA. All 9 workers had elevated IgE antibody concentrations in at least one of the two tests. Of the 4 workers who complained of shortness of breath, 3 were positive for laminin-PMDA and 2 for HSA-PMDA. All of the nonexposed subjects were negative (<0.35 kU/l) for laminin-PMDA.

Conclusion: PMDA-modified laminin could provide an additional diagnostic tool for the detection of sensitization to PMDA.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Benzoates / chemistry
  • Benzoates / immunology*
  • Chemical Industry
  • Humans
  • Immunoglobulin E / blood*
  • Immunosorbent Techniques
  • Laminin / chemistry
  • Laminin / immunology*
  • Occupational Diseases / chemically induced
  • Occupational Diseases / diagnosis*
  • Occupational Exposure
  • Respiratory Hypersensitivity / diagnosis*
  • Respiratory Hypersensitivity / etiology
  • Serum Albumin / chemistry
  • Serum Albumin / immunology*


  • Benzoates
  • Laminin
  • Serum Albumin
  • pyromellitic dianhydride
  • Immunoglobulin E