Abstract
To examine how B cell receptor affinity affects clonal selection in thymus-independent type 2 (TI-2) immune responses, we produced mice with antibodies that showed a 40-fold difference in affinity for the hapten (4-hydroxy-3-nitrophenyl)acetyl (NP). The difference in the responses of high- and low-affinity B cells to NP-Ficoll was only twofold. However, in competition experiments only the high-affinity B cells responded to antigen. CD19 deficiency increased the affinity threshold of TI-2 responses, whereas Lyn deficiency enhanced clonal expansion but abrogated B cell terminal differentiation. Thus, in TI-2 immune responses, large differences in affinity produce only small differences in the intrinsic ability of B cells to respond to antigen, and selection for high-affinity clones is due to clonal competition during the earliest stages of the response.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Animals
-
Antigens, CD / immunology
-
Antigens, CD19 / immunology
-
Antigens, Differentiation, B-Lymphocyte / immunology
-
Apoptosis
-
B-Lymphocytes / immunology
-
Binding, Competitive
-
Cell Adhesion Molecules*
-
Cell Division
-
Gene Targeting
-
Haptens / immunology*
-
Immunoglobulin Heavy Chains / immunology*
-
Immunoglobulin Variable Region / immunology*
-
Lectins*
-
Mice
-
Mice, Inbred C57BL
-
Nitrophenols / immunology*
-
Phenylacetates
-
Receptors, Antigen, B-Cell / immunology*
-
Sialic Acid Binding Ig-like Lectin 2
-
T-Lymphocytes / immunology*
-
src-Family Kinases / immunology
Substances
-
Antigens, CD
-
Antigens, CD19
-
Antigens, Differentiation, B-Lymphocyte
-
Cd22 protein, mouse
-
Cell Adhesion Molecules
-
Haptens
-
Immunoglobulin Heavy Chains
-
Immunoglobulin Variable Region
-
Lectins
-
Nitrophenols
-
Phenylacetates
-
Receptors, Antigen, B-Cell
-
Sialic Acid Binding Ig-like Lectin 2
-
4-hydroxy-5-nitrophenyl acetic acid
-
lyn protein-tyrosine kinase
-
src-Family Kinases