Cdc25C interacts with PCNA at G2/M transition

Oncogene. 2002 Mar 7;21(11):1717-26. doi: 10.1038/sj.onc.1205229.


Cdc25 activates maturation promoting factor (MPF) and promotes mitosis by removing the inhibitory phosphate from the Tyr-15 of Cdc2 in human cells. In this study, we searched the interacting protein(s) of human Cdc25C using the yeast two-hybrid screen and identified proliferating cell nuclear antigen (PCNA) as an interacting partner of Cdc25C. The interaction between Cdc25C and PCNA was confirmed in vitro and in vivo. Co-immunoprecipitation analyses using human T cell line, Jurkat, further revealed that Cdc25C interacted with PCNA transiently when cells began to enter mitosis. Immunofluorescence analysis also showed that Cdc25C and PCNA were transiently co-localized in the nucleus at the beginning of M phase. Together with the previous observations of the interaction between various cdc/cyclin and PCNA, our findings strongly suggested a potential role of PCNA at the G2 to M phase transition of cell cycle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Cycle Proteins / analysis
  • Cell Cycle Proteins / physiology*
  • G2 Phase*
  • Humans
  • Jurkat Cells
  • Mitosis*
  • Proliferating Cell Nuclear Antigen / analysis
  • Proliferating Cell Nuclear Antigen / physiology*
  • Two-Hybrid System Techniques
  • cdc25 Phosphatases / analysis
  • cdc25 Phosphatases / physiology*


  • Cell Cycle Proteins
  • Proliferating Cell Nuclear Antigen
  • CDC25C protein, human
  • cdc25 Phosphatases