Characterization of a functional neuropeptide F receptor from Drosophila melanogaster

Peptides. 2002 Apr;23(4):773-80. doi: 10.1016/s0196-9781(01)00647-7.

Abstract

Potential receptors for Drosophila neuropeptide F (DmNPF) were identified in the genome database. One receptor (DmNPFR1) sequence resembled the Lymnaea NPY receptor, an invertebrate homolog of the vertebrate Y-receptor family. DmNPFR1 was cloned and tested for functionality in stably transfected mammalian CHO cells. In whole cell binding assays, DmNPF displaced 125I-NPF in a concentration-dependent manner (IC(50) = 65 nM). DmNPF inhibited forskolin-stimulated adenylyl cyclase activity similarly (IC(50) = 51 nM). Whole-mount in situ hybridization revealed that DmNPFR1 RNA is expressed in CNS and midgut of Drosophila larvae. DmNPFR1, a new invertebrate Y-receptor homolog, apparently is a functional receptor for DmNPF.

MeSH terms

  • Adenylyl Cyclases / metabolism
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • CHO Cells
  • Cricetinae
  • Drosophila Proteins / chemistry
  • Drosophila Proteins / genetics
  • Drosophila Proteins / metabolism*
  • Drosophila melanogaster / genetics
  • Drosophila melanogaster / metabolism*
  • Humans
  • In Situ Hybridization
  • Larva / anatomy & histology
  • Larva / physiology
  • Molecular Sequence Data
  • Nerve Tissue / metabolism
  • Neuropeptides / genetics
  • Neuropeptides / metabolism*
  • Phylogeny
  • Receptors, Cell Surface / chemistry
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / metabolism*
  • Receptors, Neuropeptide / chemistry
  • Receptors, Neuropeptide / genetics
  • Receptors, Neuropeptide / metabolism*
  • Receptors, Neuropeptide Y / classification
  • Receptors, Neuropeptide Y / genetics
  • Sequence Alignment

Substances

  • Drosophila Proteins
  • NPFR protein, Drosophila
  • Neuropeptides
  • Receptors, Cell Surface
  • Receptors, Neuropeptide
  • Receptors, Neuropeptide Y
  • neuropeptide F, Drosophila
  • Adenylyl Cyclases

Associated data

  • GENBANK/AF364400