Cloning and expression of class A beta-lactamase gene blaA(BPS) in Burkholderia pseudomallei

Terence K M Cheung; P L Ho; Patrick C Y Woo; K Y Yuen; P Y Chau

doi:10.1128/AAC.46.4.1132-1135.2002

Cloning and expression of class A beta-lactamase gene blaA(BPS) in Burkholderia pseudomallei

Antimicrob Agents Chemother. 2002 Apr;46(4):1132-5. doi: 10.1128/AAC.46.4.1132-1135.2002.

Authors

Terence K M Cheung¹, P L Ho, Patrick C Y Woo, K Y Yuen, P Y Chau

Affiliation

¹ Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China.

Abstract

The beta-lactamase gene blaA(BPS) in Burkholderia pseudomallei was cloned and expressed in Escherichia coli. BPS-1 is a cephalosporinase with an isoelectric point of 7.7. Sequence analysis of BPS-1 revealed conserved motifs typical of class A beta-lactamases and a relationship to the PenA (in B. cepacia) and BlaI (in Yersinia enterocolitica) lineages.

MeSH terms

Amino Acid Sequence
Animals
Anti-Bacterial Agents / pharmacology
Burkholderia pseudomallei / drug effects*
Cloning, Molecular
Gene Expression Regulation, Bacterial / genetics
Hong Kong
Humans
Kinetics
Melioidosis / microbiology
Microbial Sensitivity Tests
Molecular Sequence Data
Soil Microbiology
Thailand
beta-Lactamases / genetics*
beta-Lactams

Substances

Anti-Bacterial Agents
beta-Lactams
beta-Lactamases
blaA protein, Burkholderia pseudomallei

Associated data

GENBANK/AF326770
GENBANK/AF441327
GENBANK/AF441328
GENBANK/AF441329