TGF beta-induced Smad signaling remains intact in primary human ovarian cancer cells

Endocrinology. 2002 Apr;143(4):1174-81. doi: 10.1210/endo.143.4.8733.


Disruptions in TGF beta signaling have been implicated in various human cancers, including ovarian cancer. Our goal was to determine whether ovarian cancer cells isolated from patient ascites fluid were growth inhibited by TGF beta 1 treatment and further characterize the expression and activity profile of TGF beta/Smad signaling components in human ovarian cancer cells. We found that 9 of 10 primary cultures of ovarian cancer cells (OC2-10) were growth inhibited by 16 pM TGF beta 1. One primary ovarian cancer sample (OC1) and the established ovarian cancer cell lines CaOV3 and SkOV3 continued to grow in the presence of TGF beta 1. All cells expressed components of the TGF beta/Smad signaling pathway including TGF beta 1, T beta RI, T beta RII, Smad2, -3, -4, and Smad anchor for receptor activation. Although OC1, CaOV3, and SkOV3 are not growth inhibited by TGF beta 1, they can transmit the TGF beta 1 signal to turn on a transfected TGF beta/Smad reporter gene, p3TP.lux. In addition, all cells up-regulate the endogenous TGF beta target genes Smad7 and PAI-1. p15(Ink4B) mRNA is also up-regulated with TGF beta 1 treatment in OC2-9, whereas the p15(Ink4B) gene has been deleted in OC1, CaOV3, and SkOV3 cells. Homozygous deletion of p15(Ink4B) may account for TGF beta resistance in some populations of ovarian cancer cells. Our data demonstrate that the TGF beta/Smad signaling pathway remains functional in human ovarian cancer cells and suggest that if abnormalities exist in the cellular response of TGF beta signals, they must lie downstream of the Smad proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Northern
  • Blotting, Western
  • Cell Cycle Proteins / biosynthesis
  • Cell Cycle Proteins / genetics
  • Cell Division / drug effects
  • Cyclin-Dependent Kinase Inhibitor p15
  • Cyclin-Dependent Kinase Inhibitor p16 / biosynthesis
  • Cyclin-Dependent Kinase Inhibitor p16 / genetics
  • DNA-Binding Proteins / physiology*
  • Female
  • Genes, Reporter / genetics
  • Humans
  • Luciferases / metabolism
  • Ovarian Neoplasms / physiopathology*
  • Phosphorylation
  • Plasminogen Activator Inhibitor 1 / biosynthesis
  • Plasminogen Activator Inhibitor 1 / genetics
  • RNA Probes
  • Reverse Transcriptase Polymerase Chain Reaction
  • Serine Proteinase Inhibitors / biosynthesis
  • Serine Proteinase Inhibitors / genetics
  • Signal Transduction / physiology*
  • Trans-Activators / physiology*
  • Transfection
  • Transforming Growth Factor beta / pharmacology*
  • Tumor Cells, Cultured
  • Tumor Suppressor Proteins*
  • Up-Regulation / drug effects


  • CDKN2B protein, human
  • Cell Cycle Proteins
  • Cyclin-Dependent Kinase Inhibitor p15
  • Cyclin-Dependent Kinase Inhibitor p16
  • DNA-Binding Proteins
  • Plasminogen Activator Inhibitor 1
  • RNA Probes
  • Serine Proteinase Inhibitors
  • Trans-Activators
  • Transforming Growth Factor beta
  • Tumor Suppressor Proteins
  • Luciferases