Authentication of Panax ginseng and Panax quinquefolius using amplified fragment length polymorphism (AFLP) and directed amplification of minisatellite region DNA (DAMD)

W Y Ha; P C Shaw; J Liu; Forrest C F Yau; J Wang

doi:10.1021/jf011365l

Authentication of Panax ginseng and Panax quinquefolius using amplified fragment length polymorphism (AFLP) and directed amplification of minisatellite region DNA (DAMD)

J Agric Food Chem. 2002 Mar 27;50(7):1871-5. doi: 10.1021/jf011365l.

Authors

W Y Ha¹, P C Shaw, J Liu, Forrest C F Yau, J Wang

Affiliation

¹ Department of Biochemistry and Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China.

PMID: 11902926
DOI: 10.1021/jf011365l

Abstract

AFLP profiles characteristic to Panax ginseng and Panax quinquefolius were generated using primers E-AGG/M-CAA. P. ginseng samples from different farms in China and Korea are homogeneous genetically [similarity index (SI) = 0.88-0.99], whereas samples of P. quinquefolius from different sources are much more heterogeneous (SI = 0.64-0.96). Detailed analysis of one of the polymorphic bands in P. ginseng led to the identification of a minisatellite Pg2, which contains eight repeats of 5'-AGGACTCATCACATTGTTACTC. The minisatellite DNA was consequently used in directed amplification minisatellite region DNA analysis to authenticate the two ginsengs.

MeSH terms

Base Sequence
DNA Fingerprinting
DNA Primers
DNA, Plant / analysis*
Drugs, Chinese Herbal
Gene Amplification*
Minisatellite Repeats*
Molecular Sequence Data
Panax / genetics*
Polymerase Chain Reaction
Polymorphism, Genetic*
Quality Control

Substances

DNA Primers
DNA, Plant
Drugs, Chinese Herbal