Noladin ether, a putative novel endocannabinoid: inactivation mechanisms and a sensitive method for its quantification in rat tissues

FEBS Lett. 2002 Feb 27;513(2-3):294-8. doi: 10.1016/s0014-5793(02)02341-4.


The occurrence of the novel proposed endocannabinoid, noladin ether (2-arachidonyl glyceryl ether, 2-AGE) in various rat organs and brain regions, and its inactivation by intact C6 glioma cells, were studied. 2-AGE was measured by isotope dilution liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry, with a detection limit of 100 fmol. A compound with the same mass and chromatographic/chemical properties as 2-AGE was found in whole brain, with the highest amounts in the thalamus and hippocampus. Synthetic [(3)H]2-AGE was inactivated by intact rat C6 glioma cells by a time- and temperature-dependent process consisting of cellular uptake and partial incorporation into phospholipids. Further data suggested that 2-AGE is taken up by cells via the anandamide/2-arachidonoyl glycerol (2-AG) membrane transporter(s), and biosynthesized in a different way as compared to 2-AG.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Analysis of Variance
  • Animals
  • Brain / metabolism*
  • Cannabinoid Receptor Modulators
  • Cannabinoids / analysis
  • Cannabinoids / antagonists & inhibitors
  • Endocannabinoids
  • Glycerides / analysis*
  • Glycerides / antagonists & inhibitors
  • Male
  • Rats
  • Rats, Sprague-Dawley


  • Cannabinoid Receptor Modulators
  • Cannabinoids
  • Endocannabinoids
  • Glycerides
  • noladin ether