Cutting edge: polarized Th cell response induction by transferred antigen-pulsed dendritic cells is dependent on IL-4 or IL-12 production by recipient cells

J Immunol. 2002 Apr 1;168(7):3127-30. doi: 10.4049/jimmunol.168.7.3127.

Abstract

To assess the influence of dendritic cell (DC) production of polarizing cytokines on Th2 and Th1 development we transferred Ag-pulsed DC generated from wild-type, IL-4(-/-), or IL-12(-/-) mice into wild-type, IL-4(-/-), or IL-12(-/-) recipients. We found that DC IL-4 was not necessary for Th2 induction and that, surprisingly, DC IL-12 was not an absolute requirement for Th1 development. However, DC IL-12 production facilitated optimal Th1 response development. Critically, recipient ability to produce IL-4 or IL-12 was essential for either Th2 or Th1 development. These data help delineate the source and importance of IL-4 and IL-12 in the process of induction of polarized T cell responses by DC.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adoptive Transfer*
  • Animals
  • Antigens, Bacterial / pharmacology
  • Antigens, Helminth / pharmacology
  • Cell Differentiation / genetics
  • Cell Differentiation / immunology
  • Dendritic Cells / immunology*
  • Dendritic Cells / metabolism*
  • Dendritic Cells / transplantation
  • Interleukin-12 / biosynthesis*
  • Interleukin-12 / deficiency
  • Interleukin-12 / genetics
  • Interleukin-4 / biosynthesis*
  • Interleukin-4 / deficiency
  • Interleukin-4 / genetics
  • Lymphocyte Activation / genetics
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Th1 Cells / immunology*
  • Th2 Cells / immunology*

Substances

  • Antigens, Bacterial
  • Antigens, Helminth
  • Interleukin-12
  • Interleukin-4