Abstract
To assess the influence of dendritic cell (DC) production of polarizing cytokines on Th2 and Th1 development we transferred Ag-pulsed DC generated from wild-type, IL-4(-/-), or IL-12(-/-) mice into wild-type, IL-4(-/-), or IL-12(-/-) recipients. We found that DC IL-4 was not necessary for Th2 induction and that, surprisingly, DC IL-12 was not an absolute requirement for Th1 development. However, DC IL-12 production facilitated optimal Th1 response development. Critically, recipient ability to produce IL-4 or IL-12 was essential for either Th2 or Th1 development. These data help delineate the source and importance of IL-4 and IL-12 in the process of induction of polarized T cell responses by DC.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adoptive Transfer*
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Animals
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Antigens, Bacterial / pharmacology
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Antigens, Helminth / pharmacology
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Cell Differentiation / genetics
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Cell Differentiation / immunology
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Dendritic Cells / immunology*
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Dendritic Cells / metabolism*
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Dendritic Cells / transplantation
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Interleukin-12 / biosynthesis*
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Interleukin-12 / deficiency
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Interleukin-12 / genetics
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Interleukin-4 / biosynthesis*
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Interleukin-4 / deficiency
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Interleukin-4 / genetics
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Lymphocyte Activation / genetics
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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Th1 Cells / immunology*
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Th2 Cells / immunology*
Substances
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Antigens, Bacterial
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Antigens, Helminth
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Interleukin-12
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Interleukin-4