Molecular motors: single-molecule recordings made easy

Curr Biol. 2002 Mar 19;12(6):R203-5. doi: 10.1016/s0960-9822(02)00750-9.

Abstract

A fusion protein of kinesin and gelsolin binds a short actin filament which can be visualized using a standard fluorescence microscope. This technique has provided new insight into the mechanism of kinesin action, and in principle it can be extended to allow single-molecule assays of any protein.

Publication types

  • Review

MeSH terms

  • Gelsolin / metabolism*
  • Green Fluorescent Proteins
  • Kinesin / genetics
  • Kinesin / metabolism*
  • Luminescent Proteins / analysis
  • Luminescent Proteins / genetics
  • Luminescent Proteins / metabolism
  • Microscopy, Fluorescence / methods*
  • Molecular Biology / methods*
  • Phalloidine / chemistry
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Rhodamines / chemistry

Substances

  • Gelsolin
  • Luminescent Proteins
  • Recombinant Fusion Proteins
  • Rhodamines
  • Green Fluorescent Proteins
  • Phalloidine
  • Kinesin