Gene and Protein Expressions of Type I Collagen Are Regulated Tissue-Specifically in Rat Hyaline Cartilages in Vivo

Eur J Morphol. 2001 Jul;39(3):149-54. doi: 10.1076/ejom.39.3.149.4675.

Abstract

The present study was designed to investigate how rat hyaline cartilages at various sites in vivo express the gene and protein of type I collagen using in situ hybridization and immunohistochemistry. The gene of pro alpha 1(I) collagen was expressed by chondrocytes in articular cartilage, and the protein of type I collagen was identified in the cartilage matrix. In contrast, growth plate cartilage expressed the gene of pro alpha 1(I) collagen, but no protein of type I collagen. Neither gene nor protein of type I collagen was expressed in cartilages of trachea and nasal septum. The present study suggested that expression of type I collagen in hyaline cartilages may be regulated tissue-specifically at gene and/or protein levels.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cartilage / metabolism*
  • Collagen Type I / biosynthesis*
  • Collagen Type I / genetics*
  • DNA, Complementary / metabolism
  • Humans
  • Hyalin / metabolism*
  • Immunohistochemistry / methods
  • In Situ Hybridization / methods
  • RNA / metabolism
  • Rats
  • Rats, Wistar
  • Tissue Distribution

Substances

  • Collagen Type I
  • DNA, Complementary
  • RNA