Anticancer activity of rViscumin (recombinant mistletoe lectin) in tumor colonization models with immunocompetent mice

Anticancer Res. 2001 Nov-Dec;21(6A):3981-7.


The antitumoral and immunostimulating properties of rViscumin (recombinant mistletoe lectin) were investigated in two mouse tumor models. After intravenous inoculation with RAW-117-P or L-1 sarcoma cells in Balb/c mice, rViscumin was given s.c. at non-toxic doses ranging from 0.3 to 150 ng rViscumin/kg. One set of experiments was performed to investigate the survival of rViscumin-treated animals. Another set was carried out to analyze the effect of rViscumin treatment on the number of tumor colonies in infiltrated lungs (RAW-117P) or liver (L-1) and the activation of immune cell subsets, respectively. An overall prolonged survival time after treatment with rViscumin and a reduction in the number of tumor colonies after administration of certain rViscumin doses was observed. Immunophenotyping of the peripheral leukocytes of treated mice revealed increased numbers of T-lymphocytes, pan-NK cells and activated monocytes. The results indicate that rViscumin has antineoplastic properties and might therefore be a promising candidate in cancer therapy.

MeSH terms

  • Adjuvants, Immunologic / pharmacology*
  • Animals
  • Antineoplastic Agents / pharmacology*
  • Drug Screening Assays, Antitumor
  • Immunocompetence
  • Liver Neoplasms, Experimental / drug therapy
  • Liver Neoplasms, Experimental / immunology
  • Liver Neoplasms, Experimental / secondary
  • Lung Neoplasms / drug therapy
  • Lung Neoplasms / immunology
  • Lung Neoplasms / secondary
  • Lymphoma, Non-Hodgkin / drug therapy
  • Lymphoma, Non-Hodgkin / immunology
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Neoplasm Transplantation
  • Plant Preparations*
  • Plant Proteins*
  • Recombinant Proteins / pharmacology
  • Ribosome Inactivating Proteins, Type 2
  • Sarcoma, Experimental / drug therapy*
  • Sarcoma, Experimental / immunology
  • Sarcoma, Experimental / secondary
  • Toxins, Biological / pharmacology*
  • Tumor Cells, Cultured


  • Adjuvants, Immunologic
  • Antineoplastic Agents
  • Plant Preparations
  • Plant Proteins
  • Recombinant Proteins
  • Ribosome Inactivating Proteins, Type 2
  • Toxins, Biological
  • ribosome inactivating protein, Viscum