Expression of the human mu opioid receptor in a stable Sf9 cell line

J Biotechnol. 2002 May 9;95(2):181-7. doi: 10.1016/s0168-1656(02)00008-1.


The cDNA that encodes the human mu opioid receptor (hMOR) has been cloned and expressed in Spodoptera frugiperda (Sf9) cells using a nonlytic vector system. The coding sequence fused to the cleavable glycoprotein signal peptide gp 64, and a C-terminal histidine tag was placed under the transcriptional control of the Orgyia pseudotsugata multicapsid nucleopolyhedrosis virus immediate-early 2 (OpIE2) promoter. Transfected cells were selected using Zeocin resistance and the receptor was constitutively expressed at approximately 12000 receptors per cell. Immunofluorescence images illustrated that more than 75% of the Sf9 cells expressed hMOR at the plasma membrane. This is the first report of the constitutive and heterologous expression of a G protein-coupled receptor in a stably transfected Sf9 cell line, under the control of the OpIE2 promoter.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Baculoviridae / genetics
  • Biotechnology
  • Cell Line
  • GTP-Binding Proteins / genetics
  • Gene Expression Regulation*
  • Humans
  • Promoter Regions, Genetic / genetics
  • Receptors, Opioid, mu / genetics*
  • Spodoptera
  • Transfection


  • Receptors, Opioid, mu
  • GTP-Binding Proteins