One-step immunochromatographic assay for screening of coeliac disease

Lancet. 2002 Mar 16;359(9310):945-6. doi: 10.1016/S0140-6736(02)08046-7.

Abstract

Tissue transglutaminase is the autoantigen that elicits endomysial antibodies, which are the serological hallmarks of coeliac disease. We describe a simple, rapid immunochromatographic assay for IgA and IgG antibodies to transglutaminase, which is highly accurate for diagnosis of this disease. Results were positive for all samples from 50 untreated coeliac patients, and negative for 40 non-coeliac patients with gastrointestinal disorders. The assay seems to be a useful alternative to biopsy for mass screening for coeliac disease.

Publication types

  • Letter

MeSH terms

  • Antibodies, Monoclonal / blood*
  • Antibodies, Monoclonal / isolation & purification
  • Celiac Disease / diagnosis*
  • Celiac Disease / immunology
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Humans
  • Immunoglobulin A / blood
  • Immunoglobulin A / immunology
  • Immunoglobulin G / blood
  • Immunoglobulin G / immunology
  • Transglutaminases / blood*
  • Transglutaminases / immunology

Substances

  • Antibodies, Monoclonal
  • Immunoglobulin A
  • Immunoglobulin G
  • Transglutaminases